![]() ![]() The commercial value of saccharides in organic matter, pharmaceutical production, and energy use further reflects their importance in various applications ( Scheller and Ulvskov, 2010 Lakshminarayanan et al., 2018). Saccharides are one of the four life-building biomacromolecules that exist in all living beings, and they have attracted widespread interest due to their biological importance such as in determining organism development and influencing adaptation to various environments ( Scheller and Ulvskov, 2010). Combining the data for categorizing polysaccharide mixtures, our study establishes a single-molecule platform for polysaccharide analysis, opening a new avenue for understanding cell wall structures, and expanding polysaccharide applications. We further demonstrated the capability of this method in distinguishing arabinoxylan and glucuronoxylan in monocot and dicot plants. This device was able to detect cell wall polysaccharide xylans at concentrations as low as 5 ng/μL and discriminate xylans with hyperacetylated and unacetylated modifications. We designed a solid-state nanopore sensor supported by a free-standing SiN x membrane in fluidic cells. Here, we develop a single-molecule approach for identifying plant polysaccharides with acetylated modification levels. In the absence of an effective methodology for polysaccharide analysis that arises from compositional heterogeneity and structural flexibility, our knowledge of cell wall architecture and function is greatly constrained. Polysaccharides are important biomacromolecules existing in all plants, most of which are integrated into a fibrillar structure called the cell wall. ![]()
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